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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Gene Conversion

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

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Gene Conversion

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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

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Related Experiment Video

Updated: Jul 16, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Reverse vaccinology and genomics.

Rino Rappuoli¹, Antonello Covacci

¹IRIS, Chiron Vaccines, 53100 Siena, Italy.

Science (New York, N.Y.)

|October 25, 2003

Summary

The genomic revolution accelerates vaccine development by enabling the discovery of novel vaccine targets. This advancement is crucial for creating more effective vaccines against various diseases.

Area of Science:

Genomics
Immunology
Vaccinology

Background:

The genomic revolution has transformed biological sciences.
Understanding pathogen genomes is key to identifying vaccine candidates.

Purpose of the Study:

To highlight the impact of genomics on vaccine target discovery.
To discuss the development of novel and effective vaccines.

Main Methods:

Genomic sequencing and analysis.
Bioinformatic approaches for target identification.
In silico and in vitro validation of vaccine targets.

Main Results:

Genomic data has significantly expanded the repertoire of potential vaccine targets.
New strategies for vaccine development are emerging based on genomic insights.

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Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

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Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Published on: June 9, 2022

Related Experiment Videos

Last Updated: Jul 16, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Published on: June 9, 2022

Improved efficacy of vaccines is being achieved through target-centric approaches.

Conclusions:

The genomic revolution is a pivotal force in modern vaccinology.
Continued integration of genomic technologies will drive future vaccine innovation.
Genomic-based strategies promise enhanced protection against infectious diseases.