Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gastrulation01:56

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...
Structure of Cardiac Muscles01:13

Structure of Cardiac Muscles

Cardiac muscle, or myocardium, is a specialized type of muscle found exclusively in the heart. Its unique structural and functional characteristics enable the heart to perform its vital role of pumping blood throughout the body continuously and rhythmically. The cardiac muscle cells, or cardiomyocytes, possess an endomysium and perimysium but do not have an epimysium.
Compared to skeletal muscles, cardiac muscle cells are small and mostly have a single nucleus. Additionally, they are usually...
Fascicle Arrangement in Skeletal Muscles01:25

Fascicle Arrangement in Skeletal Muscles

Fascicles are bundles of muscle fibers in a skeletal muscle. Muscle fascicle arrangement is directly associated with the power and range of motion of various muscles. The configuration of these fascicles can vary, leading to different functional outcomes.
The four primary types of muscle based on fascicle arrangement are:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo single-cell RNA metabolic labeling resolves early transcriptional responders in the regenerating zebrafish heart.

Nature communications·2026
Same author

Generation of a prop1 knock-in zebrafish enables single-cell transcriptomics of early pituitary development.

Endocrinology·2026
Same author

Two distinct modes of Vgll4-mediated Tead regulation control organ size in zebrafish.

Communications biology·2026
Same author

Piezo1-mediated mechanohydraulic control of cell volume drives cardiac morphogenesis.

Science advances·2026
Same author

Time-resolved single-cell transcriptomics maps zebrafish heart development.

Cell reports·2026
Same author

Ex vivo imaging reveals neutrophil behaviors in the adult zebrafish heart.

Journal of cell science·2026

Related Experiment Video

Updated: Jul 8, 2026

A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey (Petromyzon marinus)
07:03

A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey (Petromyzon marinus)

Published on: June 6, 2014

A cellular framework for gut-looping morphogenesis in zebrafish.

Sally Horne-Badovinac1, Michael Rebagliati, Didier Y R Stainier

  • 1Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics, and Human Genetics, University of California, San Francisco, CA 94143, USA.

Science (New York, N.Y.)
|October 25, 2003
PubMed
Summary
This summary is machine-generated.

Zebrafish gut looping arises from asymmetric lateral plate mesoderm migration, driven by Nodal signaling. Disrupting this migration or epithelial structure inhibits gut asymmetry, revealing a cellular basis for organ laterality.

More Related Videos

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
10:21

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
10:12

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Published on: December 20, 2019

Related Experiment Videos

Last Updated: Jul 8, 2026

A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey (Petromyzon marinus)
07:03

A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey (Petromyzon marinus)

Published on: June 6, 2014

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
10:21

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
10:12

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Published on: December 20, 2019

Area of Science:

  • Developmental biology
  • Organogenesis
  • Zebrafish model system

Background:

  • Vertebrate organ positioning is often asymmetric, but the underlying cellular mechanisms remain unclear.
  • Left-right gene expression is known to influence organ asymmetry, but downstream cellular events are poorly understood.

Purpose of the Study:

  • To investigate the cellular basis of zebrafish gut looping and asymmetric organ positioning.
  • To determine the role of lateral plate mesoderm (LPM) migration in gut laterality.
  • To elucidate the genetic regulation of asymmetric tissue movements during organogenesis.

Main Methods:

  • Utilized zebrafish as a model organism to study gut looping.
  • Investigated the effects of mutations disrupting LPM epithelial structure on gut asymmetry.
  • Performed endoderm ablation experiments to assess LPM's autonomous role in migration.
  • Manipulated Nodal signaling to observe its impact on LPM migration and gut looping patterns.

Main Results:

  • Zebrafish gut looping is driven by the asymmetric migration of the adjacent lateral plate mesoderm (LPM).
  • Disruptions in LPM epithelial integrity impede asymmetric migration and consequently inhibit gut looping.
  • LPM can autonomously generate the force for gut displacement, independent of the endoderm.
  • Reduced left-sided Nodal activity leads to randomized LPM migration and gut looping.

Conclusions:

  • Asymmetric LPM migration is a key cellular mechanism underlying zebrafish gut looping.
  • The epithelial structure of the LPM is crucial for directed cell migration and organ asymmetry.
  • Nodal signaling plays a critical role in regulating the directional migration of LPM, establishing organ laterality.