Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Toward artificial developmental regulators.

Hans-Dieter Arndt1, Karl E Hauschild, David P Sullivan

  • 1California Institute of Technology, Division of Chemistry and Chemical Engineering, Pasadena, California 91125, USA.

Journal of the American Chemical Society
|October 30, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BRD4 recruitment desilences transcription without erasure or depletion of repressive chromatin.

bioRxiv : the preprint server for biology·2026
Same author

Label-Free High-Throughput Screening of CYP3A4 Inhibitors Using Acoustic Ejection Mass Spectrometry.

Analytical chemistry·2026
Same author

Platelet Endothelial Cell Adhesion Molecule-Dependent Leukocyte Transmigration Is an Essential Early Event in Endotoxin-Induced Uveitis.

The American journal of pathology·2026
Same author

Toward development of a dynamic supramolecular peptide therapy for acute ischemic stroke.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2026
Same author

Utilizing EdU to Track Leukocyte Recruitment to the Brain.

Bio-protocol·2025
Same author

Direct targeting and regulation of RNA polymerase II by cell signaling kinases.

Science (New York, N.Y.)·2025
Same journal

A Ni-Mediated Cross-Coupling Approach to Deuterated <sup>18</sup>F- Fluoromethylated (Hetero)arenes.

Journal of the American Chemical Society·2026
Same journal

Efficient Light-Driven CO<sub>2</sub> Capture and Reversible Release Enabled by Metastable Photoacid-Decorated Metal-Organic Frameworks.

Journal of the American Chemical Society·2026
Same journal

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026
Same journal

Solvent Esterification and Stoichiometric Control in Ambient-Grown FAPbI<sub>3</sub> Single-Crystal Solar Cells.

Journal of the American Chemical Society·2026
Same journal

Unlocking Azulene Functionalization via Strain-Induced Azulyne Intermediates.

Journal of the American Chemical Society·2026
Same journal

An Oxazine-Locked Covalent Organic Framework by a Tandem Pinner/Schiff Base Reaction for Hydrogen Peroxide Photosynthesis.

Journal of the American Chemical Society·2026
See all related articles

Researchers designed a polyamide-peptide molecule that binds DNA and recruits the Exd protein. This engineered molecule acts as a protein-DNA dimerizer, enhancing developmental regulator binding efficiency.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Chemical Biology

Background:

  • The Drosophila Hox protein cofactor Exd plays a crucial role in developmental regulation.
  • Natural Hox protein partners exhibit cooperative DNA binding, but their efficiency can be improved.
  • Understanding protein-DNA interactions is key to deciphering developmental pathways.

Purpose of the Study:

  • To design a novel molecule that can specifically recruit the Exd protein to a DNA site.
  • To create a cooperative protein-DNA dimerizer that mimics natural developmental regulators.
  • To enhance the binding efficiency of Exd to its cognate DNA sequence.

Main Methods:

  • Synthesis of an eight-ring hairpin polyamide conjugated with a heptapeptide.
  • Characterization of the polyamide-peptide conjugate's binding properties.

Related Experiment Videos

  • In vitro analysis of the cooperative binding between the conjugate, Exd, and DNA.

    • Main Results:

    • The polyamide-peptide conjugate specifically recruits the Exd protein to a cognate DNA site.
    • The conjugate induced cooperative binding of Exd with a dissociation constant (Kd) of 4.4 nM.
    • This binding was an order of magnitude more efficient than the natural Hox protein partner Ubx.

    Conclusions:

    • The designed small molecule functions as a cooperative protein-DNA dimerizer.
    • This conjugate effectively mimics the function of natural Hox family developmental regulators.
    • The approach offers a novel strategy for modulating protein-DNA interactions in developmental processes.