Related Experiment Video
Updated: Aug 30, 2026

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
Published on: October 19, 2018
Modulations of food-derived substances on intestinal permeability in Caco-2 cell monolayers
1Applied Bioresearch Center, Research & Development, Department, Kirin Brewery Co., Ltd., Takasaki-shi, Gunma, Japan. konishiy@kirin.co.jp
Abstract:
The effects of more than 300 kinds of food extracts on intestinal permeability were investigated in Caco-2 cells with the use of model compounds: Lucifer Yellow (LY) for the paracellular pathway, Fluorescein (FC) for the monocarboxylic acid transporter-mediated pathway, and Rhodamine 123 (RH) for the p-glycoprotein-mediated efflux pathway. With several extracts of increasing or decreasing LY permeation, increasing FC or RH permeation was also observed, indicating modulation by dietary substances in several pathways for intestinal absorption.
Related Concept Videos
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Transcellular Transport of Solutes
Glucose Absorption Into the Small Intestine

