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[Rennet production by Rhizomucor miehei NRRL 3169].

D D Mariani1, G S Lorda, A P Balatti

  • 1Instituto de Investigación y Desarrollo en Microbiología y Química Aplicada, Departamento de Química, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, Uruguay 151, 6300 Santa Rosa, La Pampa, Argentina. darma@ar.inter.net

Revista Argentina De Microbiologia
|November 1, 2003
PubMed
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Researchers optimized rennet production using Rhizomucor miehei NRRL 3169. Fermentor conditions yielded superior enzyme activity (1160 SU/ml), surpassing previous findings for this crucial enzyme.

Area of Science:

  • Microbiology
  • Biotechnology
  • Enzyme Production

Background:

  • Rennet is essential in cheesemaking and other food industries.
  • Optimizing rennet production can reduce costs and improve efficiency.
  • Rhizomucor miehei is a known source of microbial rennet.

Purpose of the Study:

  • To investigate and enhance rennet production by selecting optimal strains of Rhizomucor miehei.
  • To evaluate the impact of different growth conditions and fermentation methods on enzyme yield.
  • To identify the most productive strain and optimal conditions for rennet enzyme synthesis.

Main Methods:

  • Screening of various Rhizomucor miehei strains for rennet production.
  • Cultivation in Erlenmeyer flasks (rotary shaker) and mechanically stirred fermentors.

Related Experiment Videos

  • Optimization of media composition, operational parameters (temperature, agitation), and fermentation time.
  • Enzyme activity assay using standard methods (SU/ml).

    • Main Results:

    • Rhizomucor miehei NRRL 3169 was identified as the best-performing strain.
    • Batch fermentation in mechanically stirred fermentors achieved the highest rennet yield (1160 SU/ml) at 168 hours.
    • The optimized batch process in fermentors significantly outperformed previous rennet production records.

    Conclusions:

    • Rhizomucor miehei NRRL 3169 is a highly effective strain for rennet production.
    • Mechanically stirred fermentors provide a superior environment for maximizing rennet enzyme yield.
    • The study presents a significant advancement in microbial rennet production efficiency.