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In vitro immune modulation of polymorphonuclear leukocyte adhesiveness by sodium fluoride
J L Gómez-Ubric1, J Liébana, J Gutiérrez
1Department of Microbiology, School of Odontology, University of Granada, Spain.
Abstract:
We investigated the influence of sodium fluoride on polymorphonuclear leukocyte (PMN) adhesiveness in a healthy subject with low serum levels of fluoride. The PMN were separated from venous blood, and the percentages of adhered and unadhered cells were determined in vitro in plastic culture plates. The cells were cultured with five different fluoride concentrations ranging from 6.25 10(-2) microM to 4.0 microM in the presence and absence of autologous serum. PMN adhesiveness in both the presence and in the absence of autologous serum was 98.5%; the addition of fluoride had no effect on the results in the absence of serum. However, in the presence of autologous serum, PMN adhesiveness decreased significantly with the addition of fluoride (P < 0.05) from 0.5 microM. The decrease was smaller (1.1%) at a concentration of 0.125 microM, and larger at 12 times this concentration of fluoride (52.7%). We conclude that sodium fluoride reduces PMN adhesiveness in a dose-dependent manner. The effect is not direct, but should be modulated by a seric factor.
Insights
Sodium fluoride was found to decrease polymorphonuclear leukocyte (PMN) adhesiveness in a dose-dependent manner, but only when autologous serum was present. This suggests a serum factor modulates fluoride
Area of Science:
- Immunology
- Toxicology
- Biochemistry
Background:
- Polymorphonuclear leukocytes (PMNs) are crucial immune cells.
- Fluoride exposure can impact biological processes.
- Understanding fluoride's effect on immune cells is important for public health.
Purpose of the Study:
- To investigate the in vitro effect of sodium fluoride on PMN adhesiveness.
- To determine if serum influences fluoride's impact on PMN function.
Main Methods:
- Human PMNs were isolated from peripheral blood.
- Cells were exposed to varying concentrations of sodium fluoride (6.25 x 10(-2) microM to 4.0 microM).
- PMN adhesiveness was measured in the presence and absence of autologous serum.
Main Results:
- Sodium fluoride did not affect PMN adhesiveness in the absence of serum.
- In the presence of autologous serum, PMN adhesiveness significantly decreased (P < 0.05) starting from 0.5 microM fluoride.
- The reduction in adhesiveness was dose-dependent, with a 52.7% decrease at the highest tested concentration.
Conclusions:
- Sodium fluoride reduces PMN adhesiveness in a dose-dependent manner.
- The effect of sodium fluoride on PMN adhesiveness is not direct and appears to be modulated by a factor present in serum.