Related Experiment Video

Updated: Aug 30, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Proteomic approaches for the identification of cell cycle-related drug targets

Mark R Flory1, Ruedi Aebersold

  • 1Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.

Progress in Cell Cycle Research
|November 5, 2003
PubMed

Insights

This study identifies proteins affected by cell cycle drugs using advanced mass spectrometry. This helps understand cancer mechanisms and develop new cancer growth-inhibiting strategies.

Area of Science:

  • Proteomics
  • Cancer Biology
  • Pharmacology

Background:

  • Cell cycle drugs offer insights into cancer mechanisms and potential treatments for uncontrolled cell growth.
  • Understanding drug targets, both direct and indirect protein interactions, is crucial for evaluating drug efficacy.

Purpose of the Study:

  • To identify the comprehensive set of proteins affected by cell cycle-modulating drugs.
  • To establish a proteome-wide profile of drug-induced changes in protein abundance and phosphorylation.

Main Methods:

  • Utilized stable isotope protein tagging for precise protein quantification.
  • Employed chromatographic separation and tandem mass spectrometry for deep proteome analysis.
  • Integrated advanced data processing for identifying affected proteins and their post-translational modifications.

Main Results:

  • Successfully identified numerous proteins directly and indirectly impacted by the studied drugs.
  • Generated a detailed proteome-wide map of altered protein levels and phosphorylation states.
  • Demonstrated the power of the integrated technology platform for comprehensive drug effect profiling.

Conclusions:

  • The integrated proteomic approach provides a powerful means to understand drug mechanisms of action.
  • This methodology aids in identifying novel therapeutic targets and strategies for cancer treatment.
  • Characterizing drug-induced proteome changes is essential for advancing cancer drug development.

More Related Videos

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Analysis of Cell Cycle Position in Mammalian Cells12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Related Concept Videos

Pharmacogenomics: Identification of New Drug Targets01:29

Pharmacogenomics: Identification of New Drug Targets

Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.