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Updated: Aug 30, 2026

Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana
Published on: January 16, 2021
[Biosynthesis of mini-antibodies to human ferritin in plant and bacterial producers]
E G Semeniuk1, O A Stremovskiĭ, I V Orlova
1Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia. centaurea@mail.ru
Abstract:
A recombinant scFv antibody against human spleen ferritin was expressed as a barstar-fused protein in Escherichia coli and in Nicotiana tabacum plants and suspension cell cultures. As demonstrated by immunoblotting with antibarstar antibodies, direction of the recombinant protein to the endomembrane system of plant cells ensured its stability and solubility. Production of the recombinant protein did not differ between parental transgenic plants and their first-generation progeny. Fusion with barstar allowed not only immunochemical detection of the recombinant scFv antibody, but also their purification from the plant material by affinity chromatography with barnase-His6 immobilized on a metal-affinity carrier.
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