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Initiation on chromatin templates in a yeast RNA polymerase II transcription system
Y Lorch1, J W LaPointe, R D Kornberg
1Department of Cell Biology, Stanford University School of Medicine, California 94305.
Abstract:
Templates were prepared with either the TATA box or transcription start sites of the yeast CYC1 promoter in a nucleosome. In both cases, initiation in an unfractionated yeast RNA polymerase II transcription system was abolished by the nucleosome. The inhibition appeared to be relieved by the activator protein Gal4-VP16 binding to a site upstream of the promoter. Inhibition was not relieved, however, in a transcription system reconstituted from purified components, indicating a requirement for additional factors for the effect of Gal4-VP16.
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