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Determination of aristolochic acids by capillary electrophoresis
Horacio A Priestap1, Silvia L Iglesias, Martín F Desimone
1Cátedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Abstract:
A method for the separation and quantification of aristolochic acids by capillary electrophoresis is described. Buffer solutions composed of sodium dihydrogen phosphate, sodium borate, and sodium dodecyl sulfate at pH 6.5-7 were found to be suitable for the separation of aristolochic acids, which can be well resolved in a few minutes. The separation and identification of six aristolochic acids contained in a commercial tincture called charrua, a traditional herbal medicine consisting of a hydroalcoholic extract of Aristolochia argentina, corroborate the usefulness of the method. The eventual toxic properties of the aristolochic acid containing charrua tincture are discussed.
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