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Molecular typing of Mannheimia (Pasteurella) haemolytica serotype A1 isolates from cattle in Japan

K Katsuda1, M Kohmoto, K Kawashima

  • 1Environmental Hygiene Section, Shichinohe Research Unit, National Institute of Animal Health, 31 Uminai, Shichinohe, Kamikita, Aomori 039-2586, Japan.

Epidemiology and Infection
|November 5, 2003
PubMed

Insights

Molecular typing of Mannheimia (Pasteurella) haemolytica serotype A1 in Japan revealed diverse genetic profiles. These diverse strains have spread across the country, potentially causing sporadic infections.

Area of Science:

  • Veterinary Microbiology
  • Molecular Epidemiology
  • Bacterial Pathogenesis

Background:

  • Mannheimia (Pasteurella) haemolytica serotype A1 is an important bovine respiratory disease pathogen.
  • Understanding the genetic diversity and distribution of M. haemolytica A1 is crucial for disease control.

Purpose of the Study:

  • To perform molecular typing of M. haemolytica A1 isolates from Japan using Pulsed Field Gel Electrophoresis (PFGE) and Random Amplified Polymorphic DNA (RAPD).
  • To investigate the genetic diversity and population structure of M. haemolytica A1 in Japan.

Main Methods:

  • DNA extraction from 130 M. haemolytica A1 isolates collected from 13 Japanese prefectures.
  • Molecular typing using ApaI PFGE and RAPD analysis.
  • Cluster analysis of PFGE profiles.

Main Results:

  • Fifteen ApaI PFGE profiles were identified, forming six distinct clusters (A-F), with cluster B being the most prevalent (40.7%).
  • Subtyping of cluster B revealed seven subtypes, with B5 being the most common (63%).
  • RAPD analysis yielded four banding patterns, with type I comprising 60.7% of isolates; RAPD type I isolates were associated with PFGE clusters A-C.

Conclusions:

  • M. haemolytica A1 isolates in Japan exhibit significant molecular diversity.
  • No correlation was found between molecular type and geographic origin, suggesting widespread dissemination.
  • The diverse and widespread nature of these strains may contribute to sporadic disease outbreaks.

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