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Updated: Aug 30, 2026

In Situ Hybridization for the Precise Localization of Transcripts in Plants
Published on: November 23, 2011
A novel feature of microsatellites in plants: a distribution gradient along the direction of transcription
Shigeo Fujimori1, Takanori Washio, Kenichi Higo
1Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0035, Japan.
Abstract:
A computer-based analysis was conducted to assess the characteristics of microsatellites in transcribed regions of rice and Arabidopsis. In addition, two mammals were simultaneously analyzed for a comparative analysis. Our analyses confirmed a novel plant-specific feature in which there is a gradient in microsatellite density along the direction of transcription. It was also confirmed that pyrimidine-rich microsatellites are found intensively near the transcription start sites, specifically in the two plants, but not in the mammals. Our results suggest that microsatellites located at high frequency in the 5'-flanking regions of plant genes can potentially act as factors in regulating gene expression.
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