Related Experiment Video
Updated: Aug 30, 2026

MALDI-ToF MS Method for the Characterization of Synthetic Polymers with Varying Dispersity and End Groups
Published on: October 3, 2025
Molecular weight determination of hypromellose phthalate (HPMCP) using size exclusion chromatography with a
1Shin-Etsu Chemical Co. Ltd., Specialty Chemicals Research Center, Kibiki-mura, Nakakubiki-gun, Niigata, Japan.
Abstract:
The molecular weight of hypromellose phthalate (HPMCP), a polymer used for enteric coating, was determined using size exclusion chromatography with a multi-angle laser light scattering detector. The values of weight-average molecular weight (Mw) of commercially available grades (HP-55, HP-55S, and HP-50) were 45600, 60200, and 37900, respectively. Their inter-day precisions expressed in terms of the coefficient of variation were less than 3%. A correlation curve between Mw and solution viscosity was prepared so that Mw could be easily estimated from the solution viscosity measured by the compendial method.
Related Concept Videos
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Determination of Molar Masses of Polymers II
High-Resolution Mass Spectrometry (HRMS)
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Types of Detectors

