Related Experiment Videos
Analytical and micropreparative separation of peptides by capillary zone electrophoresis using discontinuous buffer
1Department of Protein Chemistry, Max-Planck-Institute for Biochemistry, Martinsried, Germany.
Journal of Chromatography
|October 16, 1992
Abstract:
The tiny injection volumes that are usually necessary to maintain the high efficiency of capillary zone electrophoresis present a major problem if only limited sample amounts are available. To increase the sample load, discontinuous buffer systems were developed that allow the on-column concentration of dilute samples. Injection volumes can be increased in this way by at least a factor of 30. These stacking systems were applied to the analysis of tryptic peptides, to the purity checking of high-performance liquid chromatographic fractions and for the micropreparative separation of peptides with subsequent amino acid sequence analysis.