Related Experiment Video
Updated: Aug 30, 2026
Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
Published on: December 15, 2011
Study on metabolism of scutellarin in rats by HPLC-MS and HPLC-NMR
Jin-Lan Zhang1, Qing-Min Che, Shou-Zhuo Li
1Institute of Materia Medica, Chinese Academy of Medical Sciences 100050, China. zhjl2002by@sohu.com
Abstract:
Scutellarin is the major active constituent of Scutellaria barbata D. The metabolism of scutellarin has been investigated in rats. The solid-phase extraction and HPLC-DAD methods were established to separate and analyse metabolites. Five metabolites (M1-M5) were identified by enzymatic hydrolysis, HPLC-DAD, HPLC-MS and HPLC-NMR. M1 and M3 were conjugates of scutellarin with two sulfate groups, which have not been reported in natural plants. M2 was scutellarin; M4 was 6-methyl-scutellarin; and M5 was 6-methyl-scutellarein. The metabolic pathway was proposed.
