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Lysozyme-lysozyme and lysozyme-salt interactions in the aqueous saline solution: a new square-well potential
Bong Ho Chang1, Young Chan Bae
1Division of Chemical Engineering and Molecular Thermodynamics Lab., Hanyang University, Seoul 133-791, Korea.
Abstract:
We investigate lysozyme-lysozyme and lysozyme-salt interactions in electrolyte solutions using a molecular-thermodynamic model. An equation of state based on the statistical mechanical perturbation theory is applied to describe the interactions. The perturbation term includes a new square-well potential of mean force, which implies the information about the lysozyme surface and salt type. The attractive energy of the potential of mean force is correlated with experimental cloud-point temperatures of lysozyme in various solution conditions. The same attractive energy is used to predict osmotic pressure of a given system with no additional parameters. The new potential shows a satisfactory improvement in understanding the interactions between lysozymes in aqueous salt solutions.
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