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Creating genetically modified pigs by using nuclear transfer.

Liangxue Lai1, Randall S Prather

  • 1Department of Animal Science, University of Missouri-Columbia, Columbia, MO 65211, USA. LaiL@Missouri.edu

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Nuclear transfer (NT) technology enables the creation of genetically identical cloned pigs. This advanced method is crucial for producing gene-targeted pigs, impacting both medicine and agriculture.

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Area of Science:

  • Animal cloning and genetic engineering
  • Reproductive biotechnology
  • Swine genomics

Background:

  • Nuclear transfer (NT) is a key technique for producing genetically identical animals.
  • Recent advancements have significantly improved pig somatic NT technologies.
  • Pig cloning is essential for creating genetically modified pigs.

Purpose of the Study:

  • To review the advancements in pig somatic nuclear transfer technology.
  • To highlight the importance of NT for producing transgenic and gene-targeted pigs.
  • To discuss the potential impact of NT on medicine and agriculture.

Main Methods:

  • In vitro maturation of oocytes
  • Isolation and treatment of donor cells
  • Artificial activation of reconstructed oocytes
  • Embryo culture and transfer

Main Results:

  • Successful birth of cloned pigs in multiple laboratories.
  • Production of transgenic pigs expressing green fluorescence protein.
  • Creation of gene-targeted pigs deficient in alpha-1,3-galactosyltransferase.

Conclusions:

  • Pig somatic NT technology is transitioning from research to practical application.
  • Despite current efficiency limitations, NT is vital for specific swine genome modifications.
  • This technology is expected to significantly influence advancements in medicine and agriculture.