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HLA-B*5808, a new HLA-B allele characterized by sequence based typing
F Poli1, L Crespiatico, S Frison
1Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico IRCCS, Milano, Italy. nitpmi@policlinico.mi.it
Tissue Antigens
|November 18, 2003
Summary
A novel Human Leukocyte Antigen-B (HLA-B) allele, HLA-B*5808, was identified in an Italian donor. This discovery helps explain discrepancies between serological and molecular HLA typing methods.
Area of Science:
- Immunogenetics
- Molecular Biology
- Histocompatibility
Background:
- Accurate Human Leukocyte Antigen (HLA) typing is crucial for bone marrow transplantation.
- Discrepancies between serological and molecular typing methods can complicate donor selection.
Purpose of the Study:
- To characterize a newly identified HLA-B allele.
- To elucidate the genetic basis for conflicting HLA typing results.
Main Methods:
- Serological HLA typing.
- Molecular typing techniques.
- Direct and cloning sequencing of HLA-B exons 2, 3, and 4.
Main Results:
- A new HLA-B allele, designated HLA-B*5808, was discovered.
- HLA-B*5808 shares sequence identity with HLA-B*5801 in exon 2, differing by a silent nucleotide substitution.
- Exons 3 and 4 of HLA-B*5808 exhibit sequences characteristic of HLA-B*51, HLA-B*52, and HLA-B*78 alleles.
Conclusions:
- The unique sequence of HLA-B*5808 explains the observed discordance between serological and molecular typing.
- This finding underscores the importance of high-resolution molecular typing for complex HLA allele identification.