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[Plasmid profile of common neonatal sepsis causative agents]

M S Madaminov, I Kh Mamatkulov, M D Akhmedova

    Likars'Ka Sprava
    |November 19, 2003
    PubMed

    Insights

    Researchers analyzed plasmid profiles in Gram-negative bacteria causing neonatal sepsis. Citrobacter spp. plasmids were most common, with molecular masses ranging from 30 to 120. This aids understanding of sepsis-causing bacteria.

    Area of Science:

    • Microbiology
    • Genetics
    • Neonatal Health

    Background:

    • Neonatal sepsis is a serious health concern caused by Gram-negative bacteria.
    • Understanding the genetic elements, such as plasmids, in these bacteria is crucial for treatment and prevention.

    Purpose of the Study:

    • To investigate the plasmid profiles of Gram-negative bacterial strains responsible for neonatal sepsis.
    • To identify the prevalence of different bacterial species and their associated plasmids.

    Main Methods:

    • Plasmid DNA was isolated from 25 hospital-isolated Gram-negative bacterial strains.
    • Plasmid profiles were analyzed to determine the number, type, and molecular mass of plasmids.

    Main Results:

    • Escherichia coli, Enterobacter spp., and Citrobacter spp. were the most frequent causative agents.
    • 26 plasmids were isolated from 25 strains; 50% of these plasmids belonged to Citrobacter spp.
    • Plasmid molecular masses ranged from 30 to 120 kilobase pairs.

    Conclusions:

    • Citrobacter spp. frequently harbor plasmids that may contribute to neonatal sepsis.
    • Plasmid profiling provides insights into the genetic makeup of sepsis-causing bacteria.

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