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Published on: November 23, 2017
[Effects of As2O3 on tissue factor, plasminogen activator inhibitor-1 and -2 expression in NB4, HL-60 and THP-1
1Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To explore the effects of arsenic trioxide (As2O3) on tissue factor (TF), plasminogen activator inhibitor-1(PAI-I) and PAI-2 expression in NB4, HL-60 and THP-1 cells.
Methods:
Inhibition of the cell proliferation of NB4, HL-60 and THP-1 cells treated with As2O3 in different concentrations was observed by use of growth curves. Apoptosis was assessed by DNA electrophoresis and flow cytometric DNA contents analysis. TF, PAI-1 and PAI-2 antigen levels in cell lysates were measured by enzyme-linked immunoassay (ELISA).
Results:
1. After 24 hours exposure to 1 mumol/L As2O3 or 8 mumol/L As2O3, induced apoptosis of NB4 or HL-60 cells was noted. However induced apoptosis of THP-1 cells was not seen after exposure to 8 mumol/L As2O3 for 24 h. 2. The TF antigen levels were down-regulated during the As2O3-induced apoptosis of NB4 cells (P < 0.01), and the down-regulation effect of TF antigen levels was in the As2O3-concentration dependent fashion. Moreover, 2 mumol/L As2O3 increased the PAI-1 antigen levels in NB4 cells (P < 0.05). 3. The TF antigen levels were up-regulated and the PAI-2 antigen levels were down-regulated during the As2O3-induced apoptosis of HL-60 cells (P < 0.01 and P < 0.05, TF and PAI-2 respectively). 4. The TF and PAI-2 antigen levels in THP-1 cells were up-regulated after the exposure to 8 mumol/L As2O3 for 24 h (P < 0.05 and P < 0.01, TF and PAI-2 respectively).
Conclusion:
The effects of As2O3 on TF, PAI-1 and PAI-2 antigen levels in NB4, HL-60 and THP-1 cells are distinct.
