Related Experiment Video
Updated: Aug 30, 2026
Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
[Research for locating the mitochondrial DNA deletions of aged guinea-pigs]
Xuemei Wei1, Yuan Yang, Chuangyu Liang
1Department of Otorhinolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To locate the mtDNA deletions of aged guinea-pigs.
Methods:
By reference to the common law of mtDNA deletions in humans and rats, the possible locations of mtDNA deletions in guinea-pigs were examined by PCR and DNA sequencing. The cochlear Cortis, auditory nerve and brain tissues were harvested and the total DNA was extracted.
Results:
It was discovered for the first time that there are 4568 bps deletions of mtDNA between nt8636-nt13203 in guinea-pigs. The mtDNA deletion incidence of the aged group was significantly higher than that of the young control group (P < 0.05).
Conclusion:
There exist 4568 bps deletions between CO III and ND5 genes of mtDNA in aged guinea-pigs; mtDNA4568 deletions of guinea-pig possibly contribute to aging.
