Related Experiment Video
Updated: Aug 30, 2026

Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
Published on: November 25, 2017
[Synthesis of N-[2-(cholesteryloxycarbonylamino) ethyl] carbamoylmethylated mannan]
Li Yang1, Li Cheng, Yuquan Wei
1Key Laboratory of Biotherapy of Human Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To study the synthesis of chol-AECM-mannan.
Methods:
Using mannan as the main material, we synthesized a compound with polysaccharides partially modified by cholesterol (namely chol-AECM-mannan) through carboxymethylating, carbamoylating and condensation reaction.
Results:
The product was confirmed by IR spectrum and 1H nuclear magnetic resonance (1HNMR). The proton integral ratio of mannan and cholesterol is 100: 5, that is to say, the numbers of the substituted cholesterol per 100 mannose units are 1. 1. The production rate is 24%.
Conclusion:
The method is a effective pathway in term of synthesizing chol-AECM-mannan.
Related Concept Videos
Alkylation of β-Diester Enolates: Malonic Ester Synthesis
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Carboxylic Acids to Methylesters: Alkylation using Diazomethane
Synthesis of α-Substituted Carbonyl Compounds: The Stork Enamine Reaction
Preparation of Carboxylic Acids: Carboxylation of Grignard Reagents
Protein Glycosylation
Glycosylation occurs in...

