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'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
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A real-time fluorescence polymerase chain reaction assay for the identification of Yersinia pestis using a

James C McAvin1, Marlana A McConathy, Andrew J Rohrer

  • 1Epidemiological Surveillance Division, Molecular Epidemiology Branch, Air Force Institute for Environment, Safety, and Occupational Health Risk Analysis, Brooks Air Force Base, San Antonio, TX 78235-5237, USA.

Military Medicine
|November 19, 2003
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...