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Updated: Aug 30, 2026

Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector
Published on: July 25, 2014
Use of mathematical derivatives (time-domain differentiation) on chromatographic data to enhance the detection and
S J Ford1, M A Elliott, G W Halbert
1Cancer Research UK Formulation Unit, Department of Pharmaceutical Sciences, University of Strathclyde, 204 George Street, Glasgow, Scotland G1 1XW, UK. crcfus@strath.ac.uk
Abstract:
Two samples of an anticancer prodrug, AQ4N, were submitted for HPLC assay and showed an unidentified impurity that eluted as a 'rider' on the tail of the main peak. Mathematical derivatization of the chromatograms offered several advantages over conventional skimmed integration. A combination of the second derivative amplitude and simple linear regression gave a novel method for estimating the true peak area of the impurity peak. All the calculation steps were carried out using a widely available spreadsheet program.
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