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Related Concept Videos

Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

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Related Experiment Video

Updated: Jul 9, 2026

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

A general NMR method for rapid, efficient, and reliable biochemical screening.

Claudio Dalvit1, Elena Ardini, Maria Flocco

  • 1Chemistry and Biology Departments, Pharmacia, Viale Pasteur 10, 20014 Nerviano (MI), Italy. claudio.dalvit@pharmacia.com

Journal of the American Chemical Society
|November 20, 2003
PubMed
Summary

A new method called 3-FABS (three fluorine atoms for biochemical screening) uses fluorine-19 NMR for sensitive and efficient drug lead discovery. This technique enables high-quality screening of compound libraries and natural products for potential inhibitors.

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Area of Science:

  • Biochemistry
  • Chemical Biology
  • Drug Discovery

Background:

  • High-throughput screening is a standard method for drug-lead discovery, but assay quality is paramount.
  • Nuclear Magnetic Resonance (NMR) screening, both ligand-based and protein-based, is established for identifying compounds that bind to molecular targets.
  • Existing NMR methods offer valuable tools for biochemical screening.

Purpose of the Study:

  • To present a novel, sensitive, rapid, efficient, and reliable NMR method for biochemical screening.
  • To introduce the 3-FABS (three fluorine atoms for biochemical screening) technique for detecting enzyme activity.
  • To demonstrate the application of 3-FABS for screening compound libraries and natural product extracts.

Main Methods:

  • The 3-FABS method utilizes fluorine-19 (19F) NMR spectroscopy.
  • Substrates are labeled with a trifluoromethyl (CF3) moiety.
  • The method detects changes between starting and enzymatically modified substrates to identify active compounds.

Main Results:

  • 3-FABS enables high-quality screening of large compound and natural product collections.
  • The method allows for the determination of IC50 values for potential inhibitors.
  • Successful applications include screening for inhibitors of Ser/Thr kinase AKT1 and the protease trypsin.
  • An application in functional genomics was also demonstrated.

Conclusions:

  • The 3-FABS method provides a sensitive and efficient approach for biochemical screening.
  • This technique is valuable for drug discovery, enabling the assessment of compound libraries and natural products.
  • 3-FABS has broad applicability, including functional genomics studies.