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Published on: April 4, 2016
[Cloning and sequence analysis of Eg95 cDNA from different stages of Echinococcus granulosus in Xinjiang]
Ren-yong Lin1, Jian-bing Ding, Hao Wen
1Xinjiang Hydatid Fundamental Medical Key Lab, 1st Teaching Hospital of Xinjiang Medical University, Urumqi 830054.
Objective:
To study expression and sequence differences of Echinococcus granulosus 95(Eg95) antigen cDNA from different stages of protoscolex, oncosphere and adult worm of E. granulosus from Xinjiang Uighur Aut. Reg.
Methods:
In accordance with the sequence of Eg95 antigen cDNA, the primers of Eg95 were designed. Eg95 antigen cDNAs were amplified by PCR from protoscolex, oncosphere and adult worm cDNA libraries of E. granulosus, respectively and were cloned into pUCm-T plasmid, and sequenced. The sequences were analyzed by DNAman and GenBank/BLAST biosoftware.
Results:
PCR results showed that Eg95 antigen cDNA was amplified from three stages of E. granulosus cDNA libraries. Sequencing analysis indicated that the Eg95 cDNA length was 402 bp, same as the reported data in GenBank.
Conclusion:
The Eg95 antigen cDNA was expressed in the different life-cycle stages of E. granulosus in Xinjiang and there was no nucleic acid sequence difference of Eg95 antigen among the protoscolex, oncosphere and adult worm of E. granulosus.
