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Updated: Aug 15, 2026
Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
Published on: June 12, 2014
[Study on the effect of H2O2 against Acanthamoeba in vitro]
Qun-fei Zhao1, Xue-liang Gao, Min Qian
1State Key Laboratory of Bio-Orgnic and Natural Product Chemistry, Shanghai Institute of Orgnic Chemistry, Chinese Academy of Science, Shanghai 200032.
Objective:
To detect the effect of H2O2 on Acanthamoeba spp..
Methods:
By Wright's stain, quantitative culture, MTT assay and lactate dehydrogenase(LDH) assessment, the influence of H2O2 on the morphological feature, proliferation speed and the survival rate of Acanthamoeba was tested.
Results:
At low concentration of 0.125%, H2O2 can force the Acanthamoeba trophozoites into cysts irreversibly, and inhibit its proliferation. 1% H2O2 can directly destroy Acanthamoeba trophozoites.
Conclusion:
H2O2 is effective in destroying Acanthamoeba. It is possible to be used as an ideal reagent for the prevention of Acanthamoeba keratitis.
