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Retrofitting bacterial artificial chromosomes by homologous recombination into enhanced BAC vectors for functional

Keith al-Hasani1, Panos Ioannou

  • 1Murdoch Childrens Research Institute, University of Melbourne, Royal Children's Hospital, Melbourne, VIC, Australia.

Biotechniques
|November 25, 2003
PubMed
Abstract

No abstract available in PubMed .

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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