Callus formation and cucurbitacin B accumulation in Ecballium elaterium callus cultures
G Toker1, M Memişoğlu, M C Toker
1Gazi University, Faculty of Pharmacy, Etiler, Ankara 06330, Turkey. gulnur@gazi.edu.tr
Fitoterapia
|November 25, 2003
Abstract:
Ecballium elaterium fruit juice is used for the treatment of sinusitis in Turkish folk medicine. The aim of this study was to increase the yield of cucurbitacin B, an anti-inflammatory compound previously isolated in various organs of E. elaterium, through tissue culture techniques. Higher yields of cucurbitacin B (1.126%) were obtained from the first subculture calluses from stem nodes in the presence of benzyl adenine (BA; 1 mg/l) and naphtalene acetic acid (NAA; 0.1 mg/l) in comparison with the yields obtained from plant material (0.01%).
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