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Related Experiment Videos

BAFF/BLyS can potentiate B-cell selection with the B-cell coreceptor complex.

Hidenori Hase1, Yumiko Kanno, Masaru Kojima

  • 1Division of Immunology, Institute for Medical Science, and Department of Pathology, Dokkyo University School of Medicine, Mibu, Tochigi 321-0293, Japan

Blood
|November 25, 2003
PubMed
Summary

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Tumor necrosis factor (TNF)-like ligand BAFF enhances B-cell responses by up-regulating the CD19 coreceptor complex. This interaction is crucial for B-cell proliferation and immunoglobulin production in lymph nodes.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Tumor necrosis factor (TNF)-like ligand BAFF/BLyS is a key B-cell survival factor.
  • The functional relationship between BAFF and B-cell surface molecules like CD19 and CD40 is not well understood.
  • Follicular dendritic cells (FDCs) are important in lymph node microenvironments.

Purpose of the Study:

  • To investigate the functional relationship between BAFF and B-cell surface molecules, specifically CD19.
  • To elucidate the role of BAFF in B-cell activation and responses within the context of FDC-B-cell interactions.

Main Methods:

  • Analysis of BAFF expression on human lymph node FDCs.
  • Assessment of BAFF's effects on B-cell transcription factors (Pax5/BSAP) and CD19 expression.

Related Experiment Videos

  • Evaluation of BAFF's impact on CD19 phosphorylation, B-cell proliferation, IgG production, and reactivity to CD154 in conjunction with BCR/CD19 coligation and IL-15.

    • Main Results:

    • Follicular dendritic cells (FDCs) in human lymph nodes express BAFF abundantly.
    • BAFF up-regulates Pax5/BSAP activity and its target CD19, a component of the B-cell coreceptor complex.
    • BAFF synergistically enhances CD19 phosphorylation by the B-cell antigen receptor (BCR) and promotes B-cell proliferation, IgG production, and CD154 reactivity.

    Conclusions:

    • BAFF plays a significant role in FDC-B-cell interactions within lymph nodes.
    • BAFF influences B-cell responses through the B-cell coreceptor complex (CD19).
    • BAFF may link T cell-independent and T cell-dependent B-cell responses in germinal centers.