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Updated: Aug 30, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Effect of antioxidant probucol on cell-mediated LDL oxidation in vitro and in vivo
M V Bilenko1, A V Khil'chenko, G G Konovalova
1V. N. Orekhovich State Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow.
Abstract:
We studied the effect of phenol antioxidant probucol on free radical oxidation of LDL isolated from blood plasma of healthy donors. Oxidation was induced by co-incubation of LDL with cultured peripheral blood monocyte-macrophages and human umbilical vein endothelial cells under conditions of ischemia-reperfusion. In addition, the effect of probucol therapy on oxidability of plasma LDL in CHD patients was examined. Probucol (0.1-10 microM) efficiently protected LDL from free radical oxidation in vitro and in vivo.
