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Differential major histocompatibility complex class II locus expression on human laryngeal epithelium
L E Rees1, O Ayoub, K Haverson
1Laryngeal Research Group, Department of Otolaryngology, Division of Surgery, University of Bristol, Bristol, UK. l.e.rees@bris.ac.uk
Clinical and Experimental Immunology
|November 25, 2003
Summary
Laryngeal epithelial cells express MHC class II antigens, impacting laryngeal allograft survival. This suggests a need for immunomodulation to improve transplant outcomes.
Area of Science:
- Immunology
- Transplantation Science
- Otolaryngology
Background:
- Allograft survival is influenced by the donor organ's immunological profile, particularly Major Histocompatibility Complex (MHC) class II antigen expression.
- Antigen-presenting cells (APCs) play a crucial role in immune responses, and their expression of MHC class II dictates immune recognition.
Purpose of the Study:
- To investigate the expression levels of MHC class II antigens (HLA-DR and HLA-DQ) on laryngeal epithelial cells and other mucosal components.
- To assess the implications of these findings for laryngeal allograft survival and mucosal immune regulation.
Main Methods:
- Quantitative, multi-color immunofluorescence was employed on laryngeal and tonsillar biopsies.
- Mouse anti-human monoclonal antibodies targeting human leucocyte antigen (HLA)-DR, HLA-DQ, and CD45 were used.
Main Results:
- Laryngeal epithelium showed variable HLA-DR expression but minimal HLA-DQ expression.
- Tonsillar epithelium expressed HLA-DR in the basal layer only, with absent HLA-DQ.
- Both HLA-DR and HLA-DQ were detected on lamina propria and intraepithelial leucocytes in both tissues.
Conclusions:
- Laryngeal epithelial cells express MHC class II antigens, necessitating immunomodulation for successful laryngeal allograft transplantation.
- The low expression of HLA-DQ by laryngeal epithelium raises questions about the scope of peptide presentation and immune system response in mucosal tissues.