Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A coumermycin/novobiocin-regulated gene expression system.

Hui-Fen Zhao1, Jason Boyd, Normand Jolicoeur

  • 1Mammalian Cell Genetics, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada H4P 2R2.

Human Gene Therapy
|November 25, 2003
PubMed
Summary

Researchers developed a novel gene expression system for mammals using coumarin-activated dimerization. This system allows precise control over transgene expression, enabling rapid on/off switching for genetic studies and therapies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Suppression of certain intestinal microbiota metabolites may lead to gestational diabetes in mice fed a high-fat diet.

Frontiers in microbiology·2024
Same author

Exploring immune evasion of SARS-CoV-2 variants using a pseudotyped system.

Heliyon·2024
Same author

Recent progress in metabolic reprogramming in gestational diabetes mellitus: a review.

Frontiers in endocrinology·2024
Same author

PF-D-Trimer, a protective SARS-CoV-2 subunit vaccine: immunogenicity and application.

NPJ vaccines·2023
Same author

Real-world performance of blood-based proteomic profiling in first-line immunotherapy treatment in advanced stage non-small cell lung cancer.

Journal for immunotherapy of cancer·2021
Same author

Accuracy of reporting of Aboriginality on administrative health data collections using linked data in NSW, Australia.

BMC medical research methodology·2020

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Mammalian Systems

Background:

  • Regulated transgene expression is crucial for research and clinical applications.
  • Existing systems often lack precise on/off control or rapid switching capabilities.

Purpose of the Study:

  • To develop a novel, tightly regulated gene expression system for mammals.
  • To enable rapid, reversible control of transgene expression using small molecules.

Main Methods:

  • Constructed a transactivator by fusing bacterial DNA gyrase B subunit (GyrB) to a lambda repressor-binding domain.
  • Utilized coumarin to induce dimerization of the transactivator, activating transgene expression.
  • Employed novobiocin as an antagonist to switch off gene expression.

Related Experiment Videos

Main Results:

  • Achieved significant reduction in basal expression and a four-order-of-magnitude induction with coumermycin.
  • Demonstrated rapid on/off switching of gene expression.
  • Successfully generated stable cell lines inducibly expressing the proapoptotic bax gene.

Conclusions:

  • The coumarin-based system provides a novel method for tightly regulated gene expression in mammals.
  • This system offers rapid on/off control, expanding possibilities for functional genomics and therapeutic applications.