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Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol
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Isolation and culture of colon cancer cell lines

Jae-Gahb Park1, Ja-Lok Ku, So-Yeon Park

  • 1Cancer Research Institute, Seoul National University College of Medicine, Korea.

Methods in Molecular Medicine
|November 25, 2003
PubMed
Abstract

No abstract available in PubMed .

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Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...