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Infectious lymphocytes in mice persistently infected with lymphocytic choriomeningitis virus
Zeitschrift Fur Naturforschung. Section C, Biosciences
|November 1, 1977
Abstract:
During persistent infection of mice with the lymphocytic choriomeningitis (LCM) virus approximately 3% of leukocytes were found to contain viral antigen and to produce infectious virus. Morphologically, infected cells were shown not to be lymphoblasts and their numbers were not reduced by removal of mononuclear phagocytes. We conclude that in LCM virus carrier mice true lymphocytes participate in the infectious process.
Insights
In persistent lymphocytic choriomeningitis virus (LCMV) infections in mice, true lymphocytes, not lymphoblasts, carry the virus. This indicates lymphocytes are key participants in the infectious process.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Persistent viral infections can establish chronic reservoirs within host immune cells.
- Understanding which specific leukocytes harbor and propagate viruses is crucial for developing effective treatments.
Purpose of the Study:
- To identify the specific leukocyte populations involved in persistent Lymphocytic Choriomeningitis Virus (LCMV) infection in mice.
- To elucidate the cellular mechanisms underlying viral persistence in the host immune system.
Main Methods:
- Mice were persistently infected with LCMV.
- Leukocytes were analyzed for viral antigen presence and infectious virus production.
- Morphological analysis was performed on infected cells.
- The effect of mononuclear phagocyte depletion on infected cell numbers was assessed.
Main Results:
- Approximately 3% of leukocytes in carrier mice contained viral antigen and produced infectious LCMV.
- Morphological examination revealed that infected cells were not lymphoblasts.
- Depletion of mononuclear phagocytes did not significantly reduce the number of infected cells.
Conclusions:
- True lymphocytes, not lymphoblasts, are the primary leukocyte type involved in LCMV propagation during persistent infection.
- These findings highlight the significant role of lymphocytes in maintaining LCMV persistence.
- The results suggest that lymphocytes are critical targets for therapeutic interventions aimed at clearing persistent viral infections.