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Updated: Aug 30, 2026
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
Published on: September 2, 2014
Improved TRAP-silver staining versus conventional radioactive TRAP assays: quantification of telomerase activity
C Nemos1, J P Rémy-Martin, P Adami
1Laboratoire de Biochimie Biologie Moléculaire, Université de Franche-Comté, UFR Sciences et Techniques, Besançon, France.
Objectives:
To develop a sensitive telomeric repeat amplification protocol (TRAP)-silver staining assay for telomerase activity quantification.
Design And Methods:
TRAP assays were performed by using a TRAPeze telomerase kit with or without [alpha-32P]-dCTP. Amplification products were electrophoresed in polyacrylamide gels and detected by autoradiography or a modified silver staining protocol. Telomerase activity was quantified from radioactive counts or optical density of telomerase products from test extracts and controls.
Results:
TRAP-silver staining assay was at least as sensitive as radioactive TRAP assay and quantified telomerase activity within linearity from 10 to 3,000 cell equivalents. Both methods quantified a weak telomerase activity in normal endometrial glandular epithelial cells (GEC) and a strong increase in immortalized GEC. In human pathologic endometria (n=24), telomerase activity was correlated with lesion seriousness and distinguished simple hyperplasias from nonhyperplasic or cancerous lesions.
Conclusions:
TRAP-silver staining assay is suitable for cell and tissue telomerase activity routine quantification.
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