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Updated: Jul 23, 2026

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
Published on: March 15, 2018
Indole-3-butyric acid (IBA) production in culture medium by wild strain Azospirillum brasilense
Luis Javier Martínez-Morales1, Lucía Soto-Urzúa, Beatriz Eugenia Baca
1Centro de Investigaciones Microbiológicas, Instituto de Ciencias, Universidad Autónoma de Puebla, Apdo Postal 1622 Puebla Pue, Mexico. jlmartin@siu.buap.mx
Abstract:
Some microorganisms found in the soil are able to produce substances which regulate plant growth. In this study, we show the presence of a substance associated with auxin activity, identified as indole-3-butyric acid (IBA), in Azospirillum brasilense UAP 154 growth medium. A. brasilense was grown and indolic compounds were extracted from the supernatant. These were then analyzed by high performance liquid chromatography (HPLC), gas chromatography and gas chromatography mass spectrometry. The retention time was similar to those of the authentic IBA standard. The compound obtained from HPLC was collected and applied to maize seedlings (Zea mays), inducing biological activity along the roots, similar to that induced by an authentic IBA standard.
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