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Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
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An improved ELISA for screening for neutralizing anti-IFN-beta antibodies in MS patients

Andrew R Pachner1

  • 1Department of Neurology and Neurosciences, UMDNJ-New Jersey Medical School, Newark 07103, USA. Pachner@umdnj.edu

Neurology
|November 26, 2003
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.