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Updated: Jul 29, 2026
Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Mass spectrometric analysis of glucose-modified ribonuclease
W E Cotham1, D J S Hinton, T O Metz
1Department of Chemistry and Biochemistry, Graduate Science Research Center, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, U.S.A.
Abstract:
RNase A (1 mM) was incubated with glucose (0.4 M) at 37 degrees C for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the major sites of N epsilon-(carboxymethyl)lysine formation.
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