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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
Published on: November 11, 2014
[Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells]
Dong-mei Wang1, Li Yang, Liang Li
1Department of Stem Cell Biology, Institute of Transfusion Medicine, Beijing 100850, China.
Objective:
To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells.
Methods:
With Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank.
Results:
593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences.
Conclusion:
Part of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.
