Differentiation of Fonsecaea pedrosoi mycelial forms into sclerotic cells is induced by platelet-activating factor

Platelet-activating factor (PAF) has been shown to induce the differentiation of several cell types. In this work, we evaluated the effects of PAF on the formation of sclerotic cells of Fonsecaea pedrosoi, the major causative agent of chromoblastomycosis. Cell differentiation was evaluated by light and electron microscopy, which showed that treatment of mycelial forms with PAF results in the generation of sclerotic bodies with typical morphological characteristics. Biochemical features of PAF-induced sclerotic cells were also analyzed and compared with those from sclerotic forms induced by propranolol, a previously described differentiating agent of F. pedrosoi. Chemical analyses of lipid and carbohydrate components from PAF- or propranolol-induced sclerotic bodies revealed that palmitic, stearic, oleic and linoleic acids were the major fatty acid components, while glucose, mannose, galactose and rhamnose were detected as the principal sugar constituents in these cells. Surface carbohydrate components of PAF- and propranolol-induced sclerotic cells were also evaluated, by flow cytometry analysis with twelve different lectins. The profile of reactivity of PAF- or propranolol-induced fungal cells with lectins was also very similar. Hydrolysis of the synthetic substrate p-nitrophenylphosphate by fungal cells demonstrated that the addition of PAF or propranolol to the mycelial cultures similarly promotes a significant increase in ecto-phosphatase activity. These results indicate that the differentiation of F. pedrosoi mycelial cells induced by PAF generates authentic sclerotic forms, as confirmed by the analysis of morphological and biochemical attributes. Since PAF is synthesized in normal conditions by the human host, these observations may have a correlation with the differentiation of F. pedrosoi in vivo.