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The German cDNA network: cDNAs, functional genomics and proteomics.

Stefan Wiemann1, Stephanie Bechtel, Detlev Bannasch

  • 1Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany. s.wiemann@dkfz.de

Journal of Structural and Functional Genomics
|December 3, 2003
PubMed
Summary

Researchers are converting genomic data into protein function information. By tagging novel proteins with green fluorescent protein (GFP) and using automated assays, they are classifying proteins and assessing their effects on cell processes.

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Area of Science:

  • Genomics and Proteomics
  • Cell Biology
  • Molecular Biology

Background:

  • Exploiting vast genomic data for functional protein information is a major biological challenge.
  • Large-scale sequencing projects yield numerous novel proteins with unknown functions.

Purpose of the Study:

  • To characterize novel proteins by determining their subcellular localization.
  • To develop and implement automated functional assays for high-throughput analysis.
  • To assess the impact of novel proteins on cell growth, death, and transport.

Main Methods:

  • Tagging over 500 novel proteins with green fluorescent protein (GFP).
  • Microscopic examination of subcellular localization of GFP-fusion proteins in living cells.
  • Development and adaptation of automated functional assays for robotic platforms.

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  • Implementation of transport and proliferation assays; apoptosis and signal transduction assays in progress.

    • Main Results:

    • Proteins were classified into subcellular localization groups, guiding further functional characterization.
    • Automated assays enabled high-throughput functional screening of numerous novel proteins.
    • Identified proteins affecting cell growth, cell death, and protein transport pathways.

    Conclusions:

    • Subcellular localization and automated functional assays are effective strategies for characterizing novel proteins.
    • This approach facilitates the identification of proteins involved in key cellular processes.
    • Integrating data will help elucidate functional protein networks and enhance understanding of cell function.