Related Experiment Video
Updated: Aug 17, 2026

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
Published on: November 23, 2012
[DNA sequence analysis for the promoter of pyruvate oxidase gene from Streptococcus oralis]
Jincai Zhang1, Rong Zhang, Yunhui Zhang
1Department of Periodontology, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China.
Objective:
To elucidate the molecular structure of pyruvate oxidase gene promoter.
Methods:
The 1.30 kb fragment with promoter activity, amplified from upstream of Streptococcus oralis pyruvate oxidase gene (Sopox), was cloned into vector PBK-CMV. The positive transformed E. coli JM109 clone was selected, the recombinant plasmid was further identified with restriction mapping analysis. The positive recombinant plasmid was studied with sequence analysis.
Results:
After digesting the recombinant plasmid with Hind III, 1% agarose electrophoresis showed 1.30 kb fragment, which was consistent with predicted size. Sequence analysis revealed 1,350 bp.
Conclusion:
The Sopox promoter region is sequenced. Further characterization of the Sopox promoter region will elucidate the molecular mechanism of H2O2 production of streptococcus oralis.
Related Concept Videos
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
The Eukaryotic Promoter Region
Bacterial Transcription
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
The Eukaryotic Promoter Region

