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Related Experiment Videos

SCM4, a gene that suppresses mutant cdc4 function in budding yeast.

S A Smith1, P Kumar, I Johnston

  • 1Department of Biochemistry and Molecular Biology, University of Manchester, UK.

Molecular & General Genetics : MGG
|November 1, 1992
PubMed
Summary

The SCM4 gene in yeast suppresses a cell division cycle gene (CDC4). While SCM4 protein is not essential for cell division, it may interact with CDC4 and belong to a redundant protein family.

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Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Cell Cycle Regulation

Background:

  • The cell division cycle gene CDC4 in Saccharomyces cerevisiae has temperature-sensitive alleles.
  • Understanding genes that regulate cell division is crucial for comprehending cell cycle control.

Purpose of the Study:

  • To characterize the SCM4 gene and its protein product.
  • To investigate the interaction between SCM4 and CDC4.
  • To determine the essentiality of SCM4 in yeast mitosis and meiosis.

Main Methods:

  • Cloning of SCM4 using a high copy-number vector (pJDB207).
  • Analysis of SCM4 transcript levels in vivo.
  • Protein characterization, including domain structure analysis.
  • Gene disruption to assess SCM4 essentiality.

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Main Results:

  • SCM4 was cloned on a 1.8 kb BamHI fragment and increased SCM4 transcript levels 50- to 100-fold.
  • SCM4 encodes a 20.2 kDa protein with 187 amino acids and a tripartite domain structure.
  • SCM4 disruption did not affect mitosis or meiosis, suggesting functional redundancy.

Conclusions:

  • SCM4 suppresses a specific CDC4 allele, indicating potential protein interaction.
  • SCM4 is not essential for cell division in yeast.
  • SCM4 may be part of a family of functionally redundant proteins involved in cell cycle regulation.