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Development of a segmented model for a continuous electrophoretic moving bed enantiomer separation
Brian M Thome1, Cornelius F Ivory
1Department of Chemical Engineering, Washington State University, 118 Dana Hall, Pullman, Washington 99164-2710, USA.
Biotechnology Progress
|December 6, 2003
Summary
A mathematical model was developed to scale up continuous electrophoretic "moving bed" enantiomer separation. While it qualitatively predicts separation profiles, quantitative accuracy is limited, offering a framework for process analysis.
Area of Science:
- Chemical Engineering
- Separation Science
- Pharmaceutical Manufacturing
Background:
- Continuous electrophoretic "moving bed" enantiomer separation has achieved mg/h throughputs.
- Scaling this process is crucial for its application as a benchtop pharmaceutical production tool.
Purpose of the Study:
- To develop a steady-state mathematical model for scaling up continuous electrophoretic "moving bed" enantiomer separation.
- To predict process responses to variations in feed rate and counterflow velocities.
Main Methods:
- A four-region model based on hydrodynamic flows within the vortex-stabilized apparatus was developed.
- Concentration profiles were derived using the Piperoxan-sulfated beta-cyclodextrin system.
- The model evaluated the effects of regional flow rates on concentration profiles.
Main Results:
- The model qualitatively predicted concentration profile shapes and theoretical operational limits.
- Quantitative accuracy in matching actual enantiomer separation data was within 50%.
- Limitations include neglected electric field variations and competitive binding isotherms.
Conclusions:
- The developed model provides a theoretical framework for analyzing the "moving bed" electrophoretic separation process.
- It aids in understanding responses to changes in counterflow rate, feed rate, and molecular properties.
- Further refinement is needed for accurate quantitative prediction of enantiomer concentrations.