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Updated: Aug 17, 2026
Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
Published on: August 19, 2013
Comparison between mono- and bi-exponential models for reaction kinetics in the immunoradiometric assay of
J García Gómez1, J L Moreno Frigols
1Department of Physical Chemistry, Valencia Pharmacy Faculty, Radioisotope Service, Valencia University Hospital, Avda. Vicent Andrés Estellés s/n, 46110 Burjassot (Valencia), Spain.
Abstract:
This paper studies the kinetics of the antigen-antibody reactions involved in the analytical determination of neuron-specific enolase (NSE) by means of radiometric immunoassay (IRMA). For the global process, kinetics were found to be dependent on analyte and labelled antibody concentrations, such dependence fitting with the models described in previous papers. Viscosity results clearly indicate its negative influence on the direct reaction rate. Ionic strength shows noticeable but not too relevant effects, which suggests that the variation caused by the glycerol addition is not due to the influence of the dielectric constant of the solutions used. The effect of temperature shows activation parameters similar to the viscous flow energy of water, which suggests diffusion control for the global process. The analysis of the kinetic data of the experiences conducted can be explained by admitting that the antigen-antibody binding takes place through two different binding site types.
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