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Updated: Jun 23, 2026

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
Published on: April 4, 2014
[Influence of temperature on the calibration curves in IRMA for neuron-specific enolase and its physicochemical
T Peris Conejero1, R Olivares Pallerols, J L Moreno Frigols
1Servicio de Medicina Nuclear, Unidad de Radiofarmacia, Hospital Clínico Universitario de Valencia, and Departamento de Química Física, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain.
Background:
immunoradiometric assay (IRMA) is one of the principal methods used for the analytical determination of neuron specific enolase (NSE) concentration. We studied the influence of temperature on the calibration curves obtained by this method, and a physicochemical justification based on two theoretical models is proposed.
Material And Methods:
we used a commercially available RIA kit for NSE and a gamma counter. Data was analysed using Statistical software.
Results And Discussion:
activity bound to the antibody increases with temperature, producing results that are consistent with two modifications to the four parameter and Langmuir equations.
Conclusions:
the two models used successfully reproduce the results, with the adsorption model being preferable due to its greater simplicity and clearer physical significance.
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