Thermodynamic treatment of oligonucleotide duplex-simplex equilibria
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Updated: Aug 29, 2026

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
Published on: November 21, 2017
Richard Owczarzy1, Isard Dunietz, Mark A Behlke
1Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA.
Abstract:
Thermodynamic formulations have been devised to obtain Delta G degrees values directly from spectroscopic data at a fixed common temperature in nucleic acid duplex-simplex melting curves. In addition, the dependence of melting on salt concentration has been expressed in terms of a stepwise stoichiometric representation, which leads to a specific equation for the partition of the added sodium ions between the different oligonucleotide forms.
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