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Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
Published on: November 26, 2013
Effect of different matrices on physiological amino acids analysis by liquid chromatography: evaluation and
Ignacio Fernández-Fígares1, Luis Cuadros Rodríguez, Antonio González-Casado
1Estacion Experimental del Zaidin, Spanish Council for Scientific Research (CSIC), Department of Animal Nutrition, Camino del Jueves s/n, 1100 Armilla, Granada, Spain
Abstract:
Free amino acids in plasma, muscle and liver tissues were determined using the Pico-Tag method which involves HPLC and pre-column derivatization. A study of the accuracy in the determination is carried out by means of external calibration, standard-added calibration, Youden calibration and analyte recovery. It is conclusively shown that the method is affected by a proportional systematic error due to matrix effects, but not by a constant one. A new function named matrix-corrected calibration is proposed to correct for proportional bias when free analyte matrices for matrix-matched addition is unavailable.
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