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Updated: Aug 29, 2026
Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease
Published on: April 19, 2015
Impurity of recombinant adeno-associated virus type 2 affects the transduction characteristics following subretinal
Wei-Yong Shen1, Yvonne K Y Lai, Chooi-May Lai
1Centre for Ophthalmology and Visual Science, The University of Western Australia, 2 Verdun Street, Nedlands, Perth, WA 6009, Australia.
Abstract:
We recently reported that different purification methods of recombinant adeno-associated virus type 2 (rAAV2) affect the transduction characteristics following subretinal injection. In this study, we examined the roles of contaminant proteins from the HEK-293 cells and helper adenovirus, inactivation of helper adenovirus and cell stress induced by DNA-damaging agents in rAAV-mediated retinal transduction. Our results showed that contaminating factors/proteins resulting from the helper E1 deleted adenovirus are possibly responsible for efficient RPE transduction. Future studies of these factors will undoubtedly lead to development of new therapeutic approaches to PR- and RPE-specific retinal diseases.
